De Silva, G. G. E. H.Gunawardena, D. C.Kumar, N. S.Jayasinghe, L.Fujimoto, Y.2025-12-082025-12-082014-07-04Proceedings of the Peradeniya University International Research Sessions (iPURSE) - 2014, University of peradeniya, P 495978 955 589 180 613914111https://ir.lib.pdn.ac.lk/handle/20.500.14444/7099𝘈𝘦𝘨𝘭𝘦 𝘮𝘢𝘳𝘮𝘦𝘭𝘰𝘴 of the family Rutaceae is a tree of moderate size growing in tropical countries. All parts of the plant are used in traditional system of medicine for the treatment of broad range of diseases including asthma, anemia, fractures, wounds, swollen joints, high blood pressure, jaundice, diarrhea, troubles during pregnancy, typhoid, diabetes, bowel disorders, viral infections etc. Several classes of compounds have been reported from the various parts of the plant. Fruits of 𝘈. 𝘮𝘢𝘳𝘮𝘦𝘭𝘰𝘴 are edible. Ripe fruit is taken to keep body and mind cool. In a continuation of our studies towards the search for bioactive compounds from the edible fruits of Sri Lanka we investigated the chemistry and bioactivity of ripe fruits of 𝘈. 𝘮𝘢𝘳𝘮𝘦𝘭𝘰𝘴. Ripe fruits of 𝘈𝘦𝘨𝘭𝘦 𝘮𝘢𝘳𝘮𝘦𝘭𝘰𝘴 were blended and filtered. The filtrate was extracted with EtOAc and the residue was sequentially extracted with EtOAc and MeOH. TLC analysis indicated the presence of a series of same UV active compounds in the EtOAc extracts of both filtrate and the residue. The MeOH extract was found to be with some highly polar compounds. Hence, both EtOAc extracts were combined and screened for antifungal activity (against 𝘊𝘭𝘢𝘥𝘰𝘴𝘱𝘰𝘳𝘪𝘶𝘮 𝘤𝘭𝘢𝘥𝘰𝘴𝘱𝘰𝘳𝘪𝘰𝘪𝘥𝘦𝘴), antioxidant activity (against DPPH), phytotoxicity (against 𝘓𝘢𝘤𝘵𝘶𝘤𝘢 𝘴𝘢𝘵𝘪𝘷𝘢 germination), brine shrimp lethality (against 𝘈𝘳𝘵𝘦𝘮𝘪𝘢 𝘴𝘢𝘭𝘪𝘯𝘢 -amylase and lipase enzyme inhibitory assays. Preliminary investigation indicated the significant antifungal and antioxidant activity in EtOAc extract. Chromatographic separation of EtOAc extract over silica gel, sephadex LH-20, and PTLC furnished 12 compounds (1-12). Structures of these compounds were identified as psoralene (1), 8-methoxypsoralene (2), marmelide (3), xanthotoxol (4), 6-(dimethylallyl)umbeliferone (5), 6’,7’-epoxyaurepten (6), O- methylhalfordinol (7), O-isopentenylhalfordinol (8), (𝘌)-𝘕-(2-hydroxy-2-(4-methoxy phenyl)ethyl)cinnamamide (9), (𝘌)-𝘕-(2-(4-(3methylbut-2-enoyloxy)phenyl)-2-hydroxy ethyl)cinnamamide (10), (𝘌)-𝘕-(2-(-4(3-methylbut-2-enoyloxy)phenyl)-2-methoxyethyl) cinnamamide (11) and cinnamic acid (12) by detail analysis of ¹H & ¹³C NMR and MS spectral data. According to the availability, only the compounds 3, 4, 5, 7, 8, 9, 10 and 12 were subjected to bioassays and found that the compound 4 and 5 are having significant antifungal activity against 𝘊. 𝘤𝘭𝘢𝘥𝘰𝘴𝘱𝘰𝘳𝘪𝘰𝘪𝘥𝘦𝘴 and compounds 4, 5, 8, 10 and 12 having significant antioxidant activity (IC₅₀ < 31.25 ppm). Further the compounds 3, 4, 7 and 8 showed phytotoxicity activity (IC₅₀ < 62.5 ppm).enNatural SciencesFruitsAegle MarmelosBioactivityArticle