Thanaraj, V.2024-03-212024-03-212013-07-04Peradeniya University Research Sessions PURSE - 2012, Book of Abstracts, University of Peradeniya, Sri Lanka, Vol. 17, July. 4. 2012 pp.285978955589164613914111https://ir.lib.pdn.ac.lk/handle/20.500.14444/219Add one more authorPlants are used to derive antimicrobial agents as they possess several secondary metabolites that are active against many pathogens. Fungal pathogens adopt different mechanisms to circumvent the inhibitory actions of antifungal agents. Plant-derived natural products may offer potential new therapeutic antifungal agents which could act on Candida species. The objective of the study was to identify potential anti-candidal agents using Psidium guava (L), Syzygiumcumini (L) and Borasussflabelifer (L). The activity of the plant extracts were evaluated using the well diffusion assay. 20ml of molten agar was poured onto a 90mm Petri dish. 9mm wells were cut using a cork borer after seeding the plates with Candida species. Wells were loaded with 180 to 200μl of the extracts and incubated overnight for the observation of inhibitory zones. These plants showed activity against Candida species with C. albicans showing extreme resistance to B. flabelifer seed shoot extract than the rest of the isolates <formula>. However, it was sensitive to P. guava stem bark <formula>. Further, C. dubliniensis and C. Sake showed resistance to extract of Syzygiumcumini with a MIC value of <formula>. However, they were highly sensitive to B. flabelifer seed shoot extract with MIC values of <formula> and <formula>, respectively. C. rugosa showed extreme resistance to stem bark extract of S. cumini with a MIC value of > <formula>. However, it was highly sensitive to the seed shoot extract of B. flabelifer<formula>. C. guilliermondii showed resistance to seed shoot extract of B. flabelifer <formula> and was highly sensitive to P. guava stem bark extract <formula>. C. parapsilosis demonstrated sensitivity to extract of P. guava stem bark and seed shoot of B. flabelifer <formula>. P. ohmeri was highly sensitive to the extracts of seed shoot of B. flabelifer <formula>. For the first time, the current study revealed a moderate anti-candidal activity of water extract of B. flabelifer obtained at room temperature. In conclusion it can be stated that Psidium guava stem bark, Syzygiumcumini stem bark and Borasussflabelifer seed shoot extracts have considerable inhibitory activity against C. albicans and non-albicans species.en-USAntifungal activityPsidium guava (L)Syzygium cumini (L)Borasuss Flabelifer (L)CandidaArticle