Dissanayake, D. M. T. S.Jayasekara, K. B.Shihana, F.Dissanayake, D. M.2025-10-162025-10-162011-11-24Peradeniya University Research Session PURSE -2011, Proceeding and Abstracts, Vol.16, 24th November, 2011, University of Peradeniya, PP. 57https://ir.lib.pdn.ac.lk/handle/20.500.14444/5450Methaemoglobin reduction test (Brewer's test) is the most commonly used technique to screen the G6PD deficiency, which is the most common human enzyme deficiency in the world. The standard test procedure requires a large volume of blood (2 ml) and includes a longer incubation period of three hours at 37°C which makes it difficult to use in the field. The objective of this study was to develop a rapid diagnostic method by changing different parameters of methaemoglobin reduction test procedure. enabling it to be used in the field. Minimum incubation period. minimum blood volume and minimum reagent volumes needed for the reaction were tested. Dry tubes were prepared with the reagents necessary for the lowest volume of blood. A new enzyme activator was introduced to minimize the incubation time. The study results showed that minimum incubation time required for the standard test was 135 minutes, minimum blood volume needed was 0.100 ml, minimum NaN02 and glucose reagent volume required was 0.01 ml at O. 0864 mol/I. The minimum methylene blue volume was 0.01 ml at 0.2 X 105 mol/I. Results revealed that MgCI2 can be used as an enzyme activator and the incubation time needed for the test reduced up to 105 minutes. Development of strip method failed due to instability of the negative control. The standard methaemoglobin reduction test can be modified to reduce the incubation time. The development of the strip method needs improvement for the negative control.en-USMedical Laboratory ScienceAllied Health SciencePathologyMedicineClinical ToxicologyMetthemoglobin Reduction Test!Article