Senaweera, Y. T.Darmasiri, N. H.Shanuke, D. S.Molagoda, M. N.Ranasinghe, P.Jayasooriya, P. T.Samarakoon, K. W.2024-10-292024-10-292024-11-01Proceedings of the Postgraduate of Institute of Science Research Congress (RESCON) -2024, University of Peradeniya, P 1623051-4622https://ir.lib.pdn.ac.lk/handle/20.500.14444/2731The medicinal properties of Schleichera oleosa (Lour.) Oken, commonly called “Kon”, has been acknowledged and utilised in traditional medicine for its wide-ranging therapeutic effects. This study was carried out to determine the fatty acid content in S. oleosa seeds. Gas Chromatography-Mass Spectroscopy (GC-MS) was employed to identify and quantify fatty acid content, while its biological activities were determined using Alpha-amylase inhibition using the dinitrosalicylic acid (DNS) method. Anti-inflammatory activity was assessed by bovine serum albumin method for the methanolic extract, as well as for the hexane, ethyl acetate and aqueous fractions. The results were expressed as mean ± SD using GraphPad Prism 7.4 (n = 3). The results were analysed by one-way ANOVA followed by Tukey’s multiple comparison tests, and p < 0.05 was considered statistically significant. The analysis identified 13 major fatty acids, with Eicosanoic acid exhibiting the highest significant percentage (39.16 ± 5.64). 9-Octadecenoic acid, Hexadecanoic acid and 9,12-Octadecadienoic acid were also detected as 36.81% ± 2.39, 10.84% ± 1.40 and 6.98% ± 2.29, respectively. The anti-inflammatory potential of S. oleosa seed extract was significant, while no antidiabetic activity was observed against the alpha-amylase enzyme. The crude methanolic seed extract exhibited better anti-inflammatory activity at 81.68 ± 0.45 due to the presence of Eicosanoic acid and other fatty acid derivatives, with 82.34 ± 0.22 for Diclofenac as the standard. Our results suggest that S. oleosa seed extract is a promising source of natural compounds with anti-inflammatory properties, making it suitable for potential therapeutic, nutraceutical, and functional food applications.enAntidiabeticAnti-InflammatoryGas chromatography-Mass spectroscopySchleichera oleosa seed extractArticle