Prasadini, H.R.P.Senavirathne, W.A.S.H.Madhujith, T.Jayawardena, B.C.Wijesundara, S.Liyanage, R.2026-01-012026-01-012024-08-29Proceedings of the Peradeniya University International Research Sessions (iPURSE) – 2024, University of Peradeniya, P 1681391-4111https://ir.lib.pdn.ac.lk/handle/20.500.14444/7338The WHO emphasizes addressing diabetes and obesity for global health equity, highlighting plant-based traditional medicine as a low-risk, effective alternative to modern pharmaceuticals. This study aimed to evaluate the α-amylase and lipase inhibition activity (IC₅₀) of four duckweed varieties; Spirodella polyriza (SP), Lemina cf. minor (LM), Lemna perpusilla (LP), and Landoltia punctata (LaP) extracted from Water, 70% EtOH and 60% EtOH. The assessment evaluated their potential effectiveness in combating diabetes and obesity by targeting α-amylase, lipase enzymes respectively. Acarbose (anti-diabetic) and Orlistat (anti-obesity) drugs were utilized as standards. The SPSS MANOVA analysis showed significant differences (P<0.05) in the inhibition of α-amylase and lipase enzymes among solvent extractions and duckweed varieties. 70% EtOH extract showed the highest (P<0.05) α-amylase inhibition activity for both SP (0.14 ± 0.00 μg/mL) and LaP (0.14 ± 0.00 μg/mL). Water extract exhibited the greatest inhibition (P<0.05) for both LM (0.19 ± 0.00 μg/mL) and LP (0.54 ± 0.05 μg/mL). The study shows that four duckweed varieties demonstrated stronger α-amylase inhibitory activity (P<0.05) compared to the Acarbose (12.16 ± 0.10 μg/mL). The 60% EtOH extract of SP showed the highest inhibition of lipase activity (P<0.05), with an (IC₅₀ =1.39 ± 0.02 μg/mL). The 70% EtOH extracts of LM, LP and LaP also exhibited significant inhibition of lipase activity, with IC₅₀ value of 1.75 ± 0.01 μg/mL, 2.86 ± 0.02 μg/mL, and 2.62 ± 0.12 μg/mL, respectively. The inhibitory effect of the 60% EtOH extract of SP on lipase activity is comparable efficacy to Orlistat, with (IC₅₀ =1.33 ± 0.44 μg/mL). The results reveal that all examined duckweed varieties exhibit potential for diabetes management, and the 60% ethanol extract of SP has shown promise for obesity management, indicating their possible therapeutic applications. Exploring duckweed's bioactive compounds offers a promising approach to tackling global health issues related to diabetes and obesity.en-USAnti-diabeticAnti-obesityDuckweedsTraditional medicineα-amylase InhibitionPancreatic Lipase InhibitionArticle