Kumari, M. M. M. M. S.Amarasingha, T. H. P. S.Gajavathany, P.Rajeshwaran, M.Rajakaruna, R. S.Perera, P. K.2025-11-062025-11-062025-11-07Proceedings of the Postgraduate Institute of Science Research Congress (RESCON) -2025, University of Peradeniya, P 137ISSN 3051-4622https://ir.lib.pdn.ac.lk/handle/20.500.14444/6073Bovine theileriosis is a vector-borne parasitic disease that causes significant financial burden due to its detrimental effects on cattle health, particularly in Jaffna, Sri Lanka, where livestock remains an essential source of food and income. DNA pooling has not been utilised as a diagnostic strategy for 𝘛𝘩𝘦𝘪𝘭𝘦𝘳𝘪𝘢 detection in Sri Lanka with limited research available. This study aimed to utilise DNA pooling as a rapid, cost-effective technique to assess 𝘛𝘩𝘦𝘪𝘭𝘦𝘳𝘪𝘢 infection statuses of cattle herds in selected Jaffna farms. Blood samples (n = 60) and tick samples (n = 27) were collected from three cattle herds in Jaffna. Giemsa-stained blood smears were prepared from all samples for morphological analysis. Blood samples were pooled into three groups based on farm location, and molecular analysis targeting the MPSP gene was conducted on pooled and individual samples. A Chi square test was used to determine associations between prevalence and age, gender and farm locations. Microscopic analysis revealed an overall prevalence of 50.0% (30/60) for 𝘛𝘩𝘦𝘪𝘭𝘦𝘳𝘪𝘢 spp. whereas molecular analysis revealed a statistically higher prevalence (81.7%) (49/60, χ² = 13.374, 𝘱 = 0.00025). The presence of 𝘛. 𝘰𝘳𝘪𝘦𝘯𝘵𝘢𝘭𝘪𝘴 (genotype 5, 7) and 𝘛. 𝘢𝘯𝘯𝘶𝘭𝘢𝘵𝘢 were confirmed by bands at 776 bp and 785 bp, respectively. No significant association was found between prevalence and age (χ² = 2.456; 𝘱 = 0.117), gender (χ² = 0.0223; 𝘱 = 0.881) and farm locations (χ² = 1.684; 𝘱 = 0.431). Two tick species were identified: 𝘏𝘦𝘢𝘮𝘢𝘱𝘩𝘺𝘴𝘢𝘭𝘪𝘴 𝘣𝘪𝘴𝘱𝘪𝘯𝘰𝘴𝘢 (24/27: 88.9%) and 𝘙𝘩𝘪𝘱𝘪𝘤𝘦𝘱𝘩𝘢𝘭𝘶𝘴 𝘭𝘪𝘯𝘯𝘦𝘢𝘪 (3/27: 11.1%). Pooled molecular analysis detected 𝘛𝘩𝘦𝘪𝘭𝘦𝘳𝘪𝘢 spp. in all three farms with a 12-fold cost reduction compared to individual PCR, with no false positives in negative pools. These findings suggest that molecular analysis of pooled blood samples be used to cost-effectively detect 𝘛𝘩𝘦𝘪𝘭𝘦𝘳𝘪𝘢 infections in cattle herds, enabling timely treatment and improved disease control.enCattleJaffnaTheileriaTick-borne diseasesArticle