Salgadu, M. A.Ariyarathna, H. M. H. S.Bandara, T. P. M. S. D.Rajapaksha, E. I.Thalagala, S. K.Jinadasa, H. R. N.2025-11-242025-11-242014-07-04Proceedings Peradeniya University International Research Sessions (iPURSE) - 2014, University of Peradeniya, P 196978 955 589 180 613914111https://ir.lib.pdn.ac.lk/handle/20.500.14444/6967𝘊𝘭𝘰𝘴𝘵𝘳𝘪𝘥𝘪𝘶𝘮 𝘱𝘦𝘳𝘧𝘳𝘪𝘯𝘨𝘦𝘯𝘴 is a Gram-positive, anaerobic, spore-forming rod, ubiquitous bacterium in the environment and intestinal tracts of humans and animals. It is a major cause of food poisoning in humans in developed countries. Additionally, this organism may cause clinical disease manifested as tissue necrosis, septicaemia, and gas gangrene, due to disturbances in normal gut flora and gut motility. In the case described here, a 7-year old Labrador Retriever, trained for explosive detection had been treated initially with cefuroxime (750 mg, twice daily, I/V) for 3 days followed by meropenem (500 mg, twice daily, I/V) for 3 days. It had high fever and severe pancytopaenia throughout the duration of the illness. The carcase was presented for necropsy within four hours of death. Gross pathology and bone marrow cytology strongly suggested myelosuppression. Additionally, the presence of severe diffuse necrotic and emphysaematous splenitis, severe multi-focal necrotic hepatitis, severe diffuse necro-haemorrhagic myelitis and severe acute multi-focal follicular uro-cystitis strongly suggested death due to septicaemia, secondary to severe myelosuppression. Further, direct smears from bone marrow revealed large numbers of Gram-positive rods with a clearly demarcated halo suggesting 𝘊. 𝘱𝘦𝘳𝘧𝘳𝘪𝘯𝘨𝘦𝘯𝘴 sepsis. A pure culture of anaerobic Gram-positive rods with double zone of haemolysis was recovered from bone marrow. The identity of the organism was confirmed as 𝘊. 𝘱𝘦𝘳𝘧𝘳𝘪𝘯𝘨𝘦𝘯𝘴 using conventional biochemical tests. Even though it is a commensal, 𝘊. 𝘱𝘦𝘳𝘧𝘳𝘪𝘯𝘨𝘦𝘯𝘴 can cause occasional severe acute fatal disease in animals and humans. However, 𝘊. 𝘱𝘦𝘳𝘧𝘳𝘪𝘯𝘨𝘦𝘯𝘴-associated enteric disease in dogs is not well- characterised, but may range from mild and self-limiting to fatal acute haemorrhagic diarrhoea. Further, in this case sufficient information is not available to determine the cause of bone marrow suppression and the origin of infection, both of which might have been induced by prolonged usage of certain antibiotics including meropenem. Interestingly, it has been reported in humans that 𝘊. 𝘱𝘦𝘳𝘧𝘳𝘪𝘯𝘨𝘦𝘯𝘴 can cause acute cystitis and sepsis in bone marrow-suppressed patients. Severe 𝘊. 𝘱𝘦𝘳𝘧𝘳𝘪𝘯𝘨𝘦𝘯𝘴 infections have been reported from broiler flocks in Sri Lanka, but according to our knowledge, there are no previous reports of fatal 𝘊. 𝘱𝘦𝘳𝘧𝘳𝘪𝘯𝘨𝘦𝘯𝘴 infections from dogs in Sri Lanka. Therefore further studies are needed to determine the factors affecting the epidemiology of 𝘊. 𝘱𝘦𝘳𝘧𝘳𝘪𝘯𝘨𝘦𝘯𝘴 infection in dogs in Sri LankaenClostridium PerfringensDogsBone marrow suppressionSepticaemiaMyelosuppressionCanine pathologyArticle