Ananda, P. A. S. K.Nanayakkara, B. S.Jayasuriya, K. M. G. G.2025-11-062025-11-062025-11-07Proceedings of the Postgraduate Institute of Science Research Congress (RESCON) -2025, University of Peradeniya, P 131ISSN 3051-4622https://ir.lib.pdn.ac.lk/handle/20.500.14444/6087Although biofilms in water systems affect water quality and health, their impact on microbial contamination of residential environments is rarely studied in Sri Lanka. Microbially contaminated water and biofilms pose health risks as they can harbor pathogens and antimicrobial-resistant microorganisms. The objectives of the current study were to investigate water quality in terms of total coliforms, 𝘌𝘴𝘤𝘩𝘦𝘳𝘪𝘤𝘩𝘪𝘢 𝘤𝘰𝘭𝘪 and 𝘗𝘴𝘦𝘶𝘥𝘰𝘮𝘰𝘯𝘢𝘴 spp., to explore biofilm formation in taps and showerheads and to conduct a sociological survey on water-use at selected halls of residence of the University of Peradeniya, Sri Lanka. Fourty-eight water samples and 48 biofilm scraping samples were collected from taps and showerheads in four halls of residence: Wijewardene, Hilda Obeysekera, Ramanathan and Sarasaviuyana. Water samples were subjected to membrane filtration, and biofilm scrapings were quantified using the crystal violet assay. Biofilms were investigated using light microscopy (LM) and scanning electron microscopy (SEM). Relationship between biofilms and microbial density was detected using correlation analysis. In most samples, total coliform counts exceeded permissible limits for drinking water, according to WHO (0 CFU/100 mL) and SLSI (3 CFU/100 mL) standards, while 𝘌. 𝘤𝘰𝘭𝘪 showed localised contamination in Hilda Obeysekera (800 CFU/100 mL) and Sarasaviuyana (133 CFU/100 mL) halls, with low levels elsewhere (0 – 2 CFU/100 mL). No shower water samples exceeded permissible limits for recreational water (235 CFU of 𝘌. 𝘤𝘰𝘭𝘪/100 mL). 𝘗𝘴𝘦𝘶𝘥𝘰𝘮𝘰𝘯𝘢𝘴 spp. was not detected in any sample. In the crystal violet assay, biofilm formation showed significant differences (𝘱 < 0.05) across Halls, with Sarasaviuyana Hall showing the highest (0.343±0.046). Biofilms could be detected through LM and SEM. A weak negative correlation (𝘳 = -0.32) was observed between biofilm formation and total coliform density, suggesting that biofilms influence the presence of coliforms in water. According to the sociological survey, inadequate maintenance and irregular cleaning are possible contributors to contamination. These findings underscore the need to improve water quality management in residential halls.enCrystal violet biofilm assayEscherichia coliMembrane filtrationSociological surveyTotal coliformsArticle