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Browsing Other University Publications by Author "Amara Siri, V. H. N. H."

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    Green synthesis of Monsoon longifolium leaf extract-based silver nanoparticles and antifungal potential against selected fungal pathogens of Solanum melongena
    (Postgraduate Institute of Science (PGIS), University of Peradeniya, Peradeniya, Sri Lanka, 2024-11-01) Amara Siri, V. H. N. H.; Wanigatunge, R. P.; Edirisinghe, P.
    Botanicals are extensively researched as alternatives to synthetic fungicides in plant disease management. The acetone extract of Monodon longifolium has exhibited significant antimicrobial activity in vitro. The lower stability, shorter storage life, and less efficient application of these phytochemicals compared to synthetic pesticides can pose challenges for farmers. Green synthesis of nano fungicides is considered as a substitute to address these limitations. The objective of this research was to synthesise M. longifolium based-silver nanoparticles (Ml-AgNPs) and characterize their antifungal potential against selected fungal pathogens of Solanum melongena. A solution of 1 mM silver nitrate (AgNO3) was reduced by reacting with crude M. longfolium acetone extract dissolved in Dimethyl sulfoxide (150 ppm) at pH 8 by exposing to sunlight for 6 hrs synthesized Ml-AgNPs were collected through centrifugation and washed with distilled water. The morphology and chemical composition of Ml-AgNPs were analysed using UV-visible spectroscopy, Scanning Electron Microscopy and Fourier-Transform Infrared Spectroscopy (FTIR). Their antifungal properties were assessed against Lasiodiplodia theobromae, Pseudopestalotiopsis theae and Diaporthe eugeniae, in vitro (not replicated due to limited production of Ml-AgNPs) with M. longifolium crude extract at 2000 ppm which was used as the positive control. Ml-AgNPs synthesis was confirmed by a peak at 425 nm on the UV visible spectrum. In comparison to the M. longifolium leaf extract, the FTIR spectrum of the synthesized Ml-AgNPs displayed peaks at 3353.4 cm⁻¹, 1384.4 cm⁻¹, and 1142.4 cm⁻¹, indicating the presence of hydroxyl groups, alkanes, aromatic compounds, and carbonyl groups. Also, they were spherical in shape and nearly 80 nm in size. Growth inhibitions by synthesized Ml-AgNPs (150 ppm) against L. theobromae, D. eugeniae, and P. theae were 58.8%, 78.6%, and 83.5%, respectively. The highest and the lowest growth inhibitions by the crude extract were 81.8 ± 1.5% and 63.7 ± 0.6% against P. theae and D. eugeniae, respectively. These findings highlight the potential of Ml-AgNPs for managing fungal pathogens in S. melongena. Further research is required to increase production and assess their efficacy and sustainability in field conditions.