iPURSE

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Browsing iPURSE by Author "Abayasekara, C.L."

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    Antimicrobial activity of piper betel against Candida albicans and its effects on adherence to denture acrylic surfaces
    (University of Peradeniya, Sri Lanka, 2016-11-05) Bandaranayake, A.M.S.K.; Panagoda, G.J.; Abayasekara, C.L.; Karunaratne, A.M.
    Piper betel is an important medicinal plant with its medicinal properties extending to antimicrobial activity against several pathogenic fungi and bacteria. The objective of this study was to evaluate the efficacy of Piper betel leaf extract against Candida albicans adhesion and its biofilm formation on denture acrylics. Young betel leaves (1ˢᵗ-3ʳᵈ leaf) were subjected to vacuum infiltration with ethanol. Anticandidal activity of a range of concentrations of the leaf extract (5000 - 10000 ppm) was assessed against C. albicans (ATCC 90028) and seven laboratory isolates using the agar well bioassay. Further, using a standard biofilm adhesion assay technique, the same concentrations were used to test their ability to suppress the adhesion of C. albicans on denture acrylics, as compared to a commercial denture cleanser (positive control), and sterile distilled water (negative control). The results indicated that sterile distilled water had the highest adhesion (23.75±5.3 cells per unit area) while both, the leaf extract of concentrations of 8000 - 10000 ppm and the commercial denture cleanser showed significant (p < 0.05) suppression of C. albicans adhesion on denture acrylics (less than 1 cell per unit area). There was no significant difference between adhesion in an 8000 ppm concentration extract of P. betel and the commercial denture cleanser of equal concentration, indicating that betel leaf extract is as efficient as the commercial denture cleanser, in suppressing the adhesion and biofilm formation of C. albicans on denture acrylic surfaces.
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    Interaction of ferrous ions and free chlorine on microbiological quality of drinking water
    (University of Peradeniya, Sri Lanka, 2015-11-06) Munasinghe, T.S.; Abayasekara, C.L.; Chandrajith, R.; Jayawardana, A.
    University of Peradeniya has a nearly 60 year’s old water distribution network that consists mainly iron pipelines, most of which are now corroded to a large extent, adding iron rust to water. The aim of this study was therefore to investigate the possible consumption of free chlorine that added in to water for disinfection by iron dissolved from pipes. If free chlorine is reduced due to iron, the available amount of free chlorine would not be sufficient for the disinfection of microorganisms in water. A laboratory simulated study was carried out to investigate the possible reaction between ferrous ion and free chlorine in water using a series of and free chlorine mixtures. The effect of the reaction on the inhibition of microorganisms were also determined by spiking Serratia spp. (a total coliform) and Escherichia coli (a fecal coliform) into known mixtures of and free chlorine. The and free chlorine contents were measured spectrophotometerically while viable microbial count was assessed using membrane filtration technique that followed a confirmation tests. Furthermore free chlorine and microbiological quality were determined periodically using 27 water samples that were collected from various points of the University network. The results of this study indicated a rapid reaction between and free chlorine. The rate of free chlorine reduction was directly proportional to the amount of in water. Although inhibition of Serratia spp. and E. coli was observed due to free chlorine in water, the effect reduced drastically with increasing in water. Serratia sp. decreased gradually with the ascending chlorine levels at levels of 0.2 and 1.0 mg/L. Total inhibition of Serratia sp. were observed at 2 and 3 mg/L of while E. coli was inhibited at levels of 1.0, 2.0 and 3.0 mg/L. The content in the water distribution network of University of Peradeniya were ranged from <0.01 mg/L to 0.64 mg/L (desired value is <0.3 mg/L), while the desired free chlorine level (0.2 mg/L) was observed only at two locations (Faculty of Veterinary Science and Meewathura treatment plant). Total coliforms were detected in samples collected from Upper Hanthana, Marcus Fernando Hall, University Temple, Science Faculty, Mars Hall and Medical Faculty, while fecal coliforms were detected only in a sample from Marcus Fernando Hall. The results of microbiological test showed that water distributed from the Kandy Municipal Council and Meewathura plants are suitable for drinking, but water distributed from the Upper Hanthana plant was not suitable for human consumption according to the World Health Organization (WHO) Standards (0/CFU per 100 ml for both total and fecal coliforms) and Sri Lankan Standards (3/CFU per 100 ml for total coliforms and 0/CFU per 100 ml for fecal coliforms). The possible reason behind the unsuitable microbiological quality of water may be related to the reaction between and free chlorine in water. It was also noted that, a high level of in water was inhibitory to microorganisms in water and remove bacteria by formation of clumps.