PURSE 2013

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https://ir.lib.pdn.ac.lk/handle/20.500.14444/59

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Browsing PURSE 2013 by Subject "Acronychia pedunculata"

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    Anti-candidal activity og Acronychia pedunculata (L. ) MIQ, AND Pogostemon heyneanus benth, evaluation of Minimum Inhibitory Concentration (MIC) of A. pedunculata (l.) MIQ and its effect on relative Cell Surface Hydrophobicity (CSH)
    (The University of Peradeniya, 2013-07-04) Rathnayake, R. M. T. P. K.; Panagoda, G. J.
    The medicinal plants, namely Acronychia pedunculata and Pogostemon heyneanus were screened for potential anti-Candida activity against five medically important standard Candida species, namely Candida albicans, Candida parapsilosis, Candida krusii, Candida tropicalis and Candida glabrata. The anti-Candida activity of A. pedunculata was determined using ethanolic bark extract after an agar well diffusion method. Subsequently anti-Candida activity of both water and ehanolic leaf extract of P. heyneanus were determined against the Candida isolates mentioned above, using the same method. Ethanolic extract of A. pedunculata showed significant activity against C. albicans, C. krusii and C. parapsilosis. Minimum Inhibitory Concentration (MIC) for C. kruseii was found to be 6400 mg/L. However, MIC for other Candida species could not be determined due to coagulation of the product as the concentration increased. Hence, further studies are needed to ascertain the MIC values of these isolates with possible modifications. Water extracts of P. heyneanus leaves showed no activity against any tested Candida species. The ethanolic extract showed significant activity against C. glabrata. Relative Cell Surface Hydrophobicity (CSH) influences the virulence of Candida isolates. In the present study, CSH was evaluated after exposing to ethanolic bark extract of A. pedunculata. An increase in hydrophobicity of C. kruseii was observed (21.63%). However, the hydrophobicity of the rest of the isolates decreased after exposure to the same compound. These results prove that A. pedunculata bark extract and P. heyneanus leaf extract have anti-Candida activity against a few of the selected Candida species and these can be used as a natural source of anti-Candida compounds. Further studies are needed involving clinical isolates of Candida species.