PURSE 2000

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https://ir.lib.pdn.ac.lk/handle/20.500.14444/46

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Browsing PURSE 2000 by Subject "Dental"

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    Effect of dexamethasone on the metabolism of testosterone in human gingival fibroblasts
    (University of Peradeniya, 2000-12-16) Tilakaratne, A.; Soory, M.
    Dexamethasone has been known to have synergistic interactions with growth factors to induce proliferation of human diploid periodontal ligament and gingival fibroblasts. It can also stimulate the formation of new cementum and alveolar bone. The anabolic effects of androgens especially 5a-dihydrotestosterone (DHT) are well established. The aim of this investigation was to study the effects of dexamethasone (Dex) on the metabolism of testosterone in human gingival fibroblasts (HGF). Confluent monolayer cultures of HGF of 5th_9'h passage derived from chronically inflamed gingival tissues were established in Eagle's Minimum Essential medium. Duplicate incubations were performed with 14C-testosterone (l4C-T) as substrate and serial concentrations of Dex ranging from 0.5-50 ug/ml for 24 hours. At the end of the incubation period. the steroid metabolites were ana lysed and quantified using a radioisotope scanner. Most of the lower concentrations of Dex showed a stimulatory effect on DHT synthesis from 14C-T. with a maximum increase of 44% at 1.0 ug/ml, decreasing to 14% at 10 ug/ml and reaching control values at higher concentrations. The increases in DHT in response to the lower concentrations of Dex (ranging from 0.5-8.0 ug/ml) were statistically significant (n=6: p<0.05: ANOYA). Synthesis of the other major metabolite. -l-andrcstenedione from 14C-T showed 20- 36% increases in response to the range of 0.5-5.0 ug/ml of De x (n=6: p<0.05: ANOYA). Dexamethasone at lower concentrations could induce the metabolism of testosterone in cultured human gingival fibroblasts resulting in the formation of anabolic metabolites. This could enhance connective tissue and bone matrix synthesis and play an important role in tissue regeneration in inflammatory periodontal lesions.
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    Is the pattern of invasion a reliable predictor of metastasis of oral cancer
    (University of Peradeniya, 2000-12-16) Siriwardena, B. S. M. S.; Tilakaratne, W. M.
    Although oral cancer accounts for over 35% of all the cancers in our patients, there is no reliable way to predict prognosis. Various studies have shown different relationship of histological features to prognosis. but the results are not conclusive. Arrangement of cells at the advancing front of the tumour may playa significant role in predicting prognosis. Metastasis is considered as a key clinical and histological parameter which decides the outcome of a given patient. Therefore the aim of this study was to compare the relationship between the pattern of invasion and metastasis. Fifty cases of oral squamous cell carcinoma which have been excised with the neck lymph nodes, received at the Department of Oral Pathology, Faculty of Dental Sciences, University of Peradeniya from July 1999 to August 2000 were selected for this study. Pattern of invasion has been graded as type 1.2.3, and 4 according to the arrangement of tumour cells at the advancing front. Statistical analysis showed metastasis of 22% in type 2. 50% in type 3, and 71% in type 4 oral squamous cell carcinoma patients. These results were statistically significant and the p value was 0.001
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    Knowledge of patients regarding their disease condition following treatment at a general hospital
    (University of Peradeniya, 2000-12-16) Kularatne, M. S. M.; Liyanagama, K. L. T. N.; Wanigasundara, P. C.
    The aim of this study was to assess the knowledge of patients regarding their disease condition following treatment at a general hospital. A convenient sample of 100 patients who were leaving the General Hospital, Peradeniya, after obtaining care were interviewed using a pre-tested structured questionnaire, to determine their awareness of the diagnosis, any special investigations that had been carried out and the treatment plan. The patients were graded into 4 categories depending on the level of knowledge they possessed. It was found that on the whole, patients had limited knowledge about various aspects of their condition. Patients with chronic conditions were better informed than those who had reported with some acute problem. There was a tendency of patients who had received more formal education having more knowledge.