In vitro antimicrobial activity of leaf exreacts of Magifera zeylanica
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Date
2013-07-04
Authors
Dhanarisi, H. K. J.
Jayatilake, J. A. M. S.
Adikaram, N. K. B.
Journal Title
Journal ISSN
Volume Title
Publisher
The University of Peradeniya
Abstract
Natural products have been used as a source for many drugs from the very dawn of medicine. With the evolution of resistance by microorganisms to present antimicrobials, the need for novel discoveries is urgent. Many researchers have demonstrated antimicrobial effects of Mangifera indica plant extracts. This study was undertaken to find out the antimicrobial activity of leaf extracts of Mangifera zeylanica which is an endemic plant in Sri Lanka.
The leaves of a Mangifera zeylanica plant (identity confirmed) were collected from the Gannoruwa forest. Leaf extracts were prepared by the maceration method using water at room temperature, water at celsius 70, boiled water and ethanol. Antimicrobial effect of the plant extract at a concentration of 25mg/ml was tested against six isolates of bacteria; Staphylococcus aureus (NTCC 6571), Escherichia coli (NTCC 10417), Pseudomonas aerugenosa (NTCC 10662), Streptococcus pyogenes, Enterococcus faecalis and Methiciline resistant Staphylococcus aureus (MRSA) and five species of Candida; Candida albicans (ATCC 90028), Candida parapsilosis (ATCC 22019), Candida krusei (ATCC 6258), Candida glabrata (ATCC 90030), Candida tropicalis (ATCC 13803) using the agar well diffusion method. Minimum inhibitory concentrations (MIC) were determined for the extracts with high activity using the agar dilution method.
When the diameter of the zones of inhibition was considered, the highest antimicrobial activity was detected against S. aureus followed by E. faecalis, E. coli and P. aerugenosa. Noticeable antimicrobial activity was detected against C. parapsilosis, followed by C. glabrata, C. krusei and C. tropicalis except C. albicans. Ethanol and celsius 70 water extracts showed higher antimicrobial activity. For the ethanol extract, the MIC value for both S. aureus and MRSA was 0.2 mg/ml. E. faecalis had a MIC value of 0.8 mg/ml. The MIC value for P. aerugenosa was 1.6 mg/ml and for E. coli MIC was 3.2 mg/ml. MIC value for C. krusei, C. prapsilosis and C. glabrata was 0.1mg/ml while the MIC value for C. tropicalis was 12.8 mg/ml. For the celsius 70 water extract, both S. aureus and MRSA showed a MIC value of 0.4 mg/ml. MIC value for E. faecalis was 1.6 mg/ml. MIC value for C. prapsilosis was 0.1mg/ml and the MIC value for C. krusei was 0.2mg/ml. C. glabrata had a MIC value of 0.8 mg/ml.
Leaf extracts of Mangifera zeylanica under different conditions, exhibit marked antimicrobial activity. Ethanol and celsius 70 water extracts are effective against many microorganisms including S. aureus, E. faecalis, P. aerugenosa and non-albicans Candida.
Description
Keywords
Health and Hygiene , Antimicrobial activity , Mangifera zeylanica
Citation
Peradeniya University Research Sessions PURSE - 2012, Book of Abstracts, University of Peradeniya, Sri Lanka, Vol. 17, July. 4. 2012 pp. 116