Antifungal effect of green synthesized zinc oxide nanoparticles against plant pathogens Fusarium spp.
| dc.contributor.author | Madusanka, H. K. S. | |
| dc.contributor.author | Aruggoda, A. G. B. | |
| dc.contributor.author | Chathurika, J. A. S. | |
| dc.contributor.author | Weerakoon, S. R. | |
| dc.date.accessioned | 2024-10-29T06:44:09Z | |
| dc.date.available | 2024-10-29T06:44:09Z | |
| dc.date.issued | 2024-11-01 | |
| dc.description.abstract | This study evaluated the antifungal potential of green-synthesized zinc oxide nanoparticles (ZnO NPs) against plant pathogens of the Fusarium species. The synthesis process utilised Mimosa pigra leaves, which were dehydrated, pulverized, and extracted by mixing 8 g of dried leaf powder with 100 mL of double-distilled water. Zinc nitrate hexahydrate (Zn(NO₃)₂·6H₂O) served as the precursor salt for the eco-friendly synthesis of ZnO NPs. The formation of ZnO NPs was confirmed through UV-visible spectroscopy, revealing a characteristic absorption peak at 356 nm. Scanning electron microscopy indicated a heterogeneous distribution of particle shapes and sizes, with an average particle size of 81 nm (range: 35-164 nm). The antifungal efficacy of the synthesized ZnO NPs was assessed using the poisoned food technique. ZnO NP concentrations of 0 (negative control), 50, 100, 500, and 1000 mg/L were incorporated into potato dextrose agar (PDA) plates. Fusarium species mycelial plugs (8 mm diameter) were inoculated into 9 cm diameter Petri dishes. All treatments were incubated at 28 ± 2 °C for seven days, with three replicates per treatment. Mycelial growth was measured, and statistical analysis was performed using one-way ANOVA, yielding a significant F-value of 43.93 (p < 0.001). Duncan’s Multiple Range Test (DMRT) was applied as a post hoc analysis to identify significant differences in growth inhibition between treatments. ZnO NPs exhibited concentration-dependent inhibition of Fusarium species, with the highest inhibition (61.52%) observed at 1000 mg/L and 22.21% inhibition at 500 mg/L compared to the control. These findings indicated that green-synthesized ZnO NPs could be a sustainable approach for managing Fusarium infections in agricultural systems. Further research is warranted to elucidate the mechanisms of action and evaluate their potential for field applications. | |
| dc.description.sponsorship | Financial assistance from The Open University of Sri Lanka (Competitive Research Grant No. 202003) is acknowledged. | |
| dc.identifier.citation | Proceedings of the Postgraduate Institute of Science Research Congress (RESCON) -2024, University of Peradeniya, P. 122 | |
| dc.identifier.issn | 3051-4622 | |
| dc.identifier.uri | https://ir.lib.pdn.ac.lk/handle/20.500.14444/2758 | |
| dc.language.iso | en | |
| dc.publisher | Postgraduate Institute of Science (PGIS), University of Peradeniya, Sri Lanka | |
| dc.relation.ispartofseries | Volume 11 | |
| dc.subject | Antifungal activity | |
| dc.subject | Fusarium species | |
| dc.subject | Green-synthesized ZnO nanoparticles | |
| dc.subject | Poisoned food technique | |
| dc.title | Antifungal effect of green synthesized zinc oxide nanoparticles against plant pathogens Fusarium spp. | |
| dc.type | Article |