Determination of antioxidant and enzyme inhibitory activities of Osbeckia octandra L., Cissus quadrangularis, and Vitex negundo in Sri Lanka

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dc.contributor.authorMallawa Arachchi, M. A. K. H.
dc.contributor.authorMendis, B. E. P.
dc.contributor.authorSiriwardhane, U.
dc.contributor.authorKalinga, J.
dc.contributor.authorPiyasena, N. P.
dc.contributor.authorMarikkar, J. M. N.
dc.contributor.authorAdikaram, N. K. B.
dc.contributor.authorJayasinghe, L.
dc.date.accessioned2024-10-29T16:20:42Z
dc.date.available2024-10-29T16:20:42Z
dc.date.issued2024-11-01
dc.description.abstractMedicinal plants have gained much attention for their therapeutic properties and diverse pharmacological effects. They are widely used in herbal healthcare practices for treating diseases. This study investigated three selected medicinal plants in Sri Lanka, traditionally used for digestive issues, bone ailments, and inflammatory conditions, to assess their in vitro bioactive potential: antioxidative, anti-hyperglycaemic, anti-obesity, and cytotoxic properties. Dried powdered samples of leaves from three medicinal plants, namely Osbeckia octandra L. (“Heen bovitiya”), Cissus quadrangularis L. (“Heeressa”), and Vitex negundo L. (“Nika”) was extracted with methanol (MeOH) via ultrasonication, followed by rotary evaporation to obtain crude extracts. The extracts were evaluated for total phenolic content (TPC) and total flavonoid content (TFC), which were separately quantified using the Folin Ciocalteu method [pyrogallol equivalent (PE)] and AlCl₃ colorimetric method [quercetin equivalent (QE)] respectively. Among the extracts, O. octandra possessed the highest TPC (291.69 ± 11.41 mg of GAE/g) and TFC (2.48 ± 0.17 mg of CE/g) values. Further, O. octandra exhibited the highest antioxidant activity in both FRAP assay (7268.00 ± 95.6 mmol FeSO4/g), positive control Trolox (13447.00 ± 19.80 mmol FeSO₄/g) and the DPPH radical scavenging assay (IC₅₀ = 11.43 ± 0.79 mg/L; positive control ascorbic acid; IC₅₀ = 3.46 ± 0.45 mg/L). Furthermore, of the three plants, only O. octandra exhibited minor inhibitory activity against α-amylase (IC₅₀ = 1129.8 ± 140.2 mg/L, positive control acarbose, IC₅₀ = 8.51 ± 0.67 mg/L). All three extracts showed significant α-glucosidase inhibitory activity at a concentration of 1000 mg/L, in which the O. octandra extract showed the highest inhibition of 99%. However, none of the extracts showed lipase inhibitory activity or brine shrimp lethality. Based on the findings, Osbeckia octandra L. demonstrated the most promising antioxidant potential among the three medicinal plants studied, which has the potential to be developed as an ingredient in functional foods and as an alternative remedy for managing non-communicable diseases.
dc.identifier.citationProceedings of the Postgraduate Institute of Science Research Congress (RESCON) -2024, University of Peradeniya, P 182
dc.identifier.issn3051-4622
dc.identifier.urihttps://ir.lib.pdn.ac.lk/handle/20.500.14444/2809
dc.language.isoen
dc.publisherPostgraduate Institute of Science (PGIS), University of Peradeniya, Peradeniya ,Sri Lanka
dc.relation.ispartofseriesVolume 11
dc.subjectAntioxidant activity
dc.subjectBioactivities
dc.subjectEnzyme inhibition
dc.subject“Heen bovitiya”
dc.subjectMedicinal plants
dc.titleDetermination of antioxidant and enzyme inhibitory activities of Osbeckia octandra L., Cissus quadrangularis, and Vitex negundo in Sri Lanka
dc.typeArticle
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