Detection of wise gene in MycobacteriumTuberculosis

Abstract

Wise (Wnt modulator In Surface Ectoderm) is a novel gene, which can modulate Wnt (Wingless-type MMTV integration site family) signaling pathway and Bone Morphogenetic Protein (BMP) signals and it is expressed in the surface ectoderm, in which Wnt signaling plays multiple roles. Wise was isolated as a secreted protein capable of interacting with Wnt co-receptor, Lipoprotein Receptor-related Protein 6 (LRP6). It either enhances or inhibits the Wnt pathways depending on the cellular context and ·it inhibits the BMP signals by binding to BMP ligands. Very little is known about the Wise cloned from mouse, Xenopus and human. Wise also called as Uterine Sensitization Associated Gene-l (USAG-l) and Ectodin. The aim of the present study was to detect Wise gene in Mycobacterium tuberculosis. M tuberculosis is the most clinically important member of the MTB complex. In this study PCR based assay was used to detect the presence of Wise gene in M tuberculosis. DNA Extraction was carried out by N-Cetyl-N-,N,N- tiymethyl ammonium bromide (CTAB) method from 50 M tuberculosis cultures. DNA amplification was performed, targeting Wise gene by using specific forward primer W2F (5'- GCTTTTAAGAATGATGCCAC-3') and reverse primer W2R (5'- GTGACCACGGTGATTTTGTA-3') Twelve strains were PCR positive along with the H37Rv standard DNA. 38 were PCR negative. With this preliminary study we can confirm the presence of Wise gene in M tuberculosis, and PCR based assay could be used to detect Wise gene in M tuberculosis. Twelve strains were PCR positive along with the H37Rv standard DNA. 38 were PCR negative. With this preliminary study we can confirm the presence of Wise gene in M tuberculosis, and PCR based assay could be used to detect Wise gene in M tuberculosis.