Bioactivity of Strychnos potatorum seeds and derived product: antioxidant, anti-inflammatory and cytotoxic properties
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Date
2024-11-01
Authors
Pathirana, G. H. P. S. S.
Herath, K. K. D. P.
Kuruppu, A. I.
Journal Title
Journal ISSN
Volume Title
Publisher
Postgraduate Institute of Science (PGIS), University of Peradeniya, Sri Lanka
Abstract
Strychnos potatorum is widely recognized for its medicinal and industrial applications due to its bioactivity, which includes antioxidant, anti-inflammatory, and anti-microbial properties. This study evaluated the bioactivity of S. potatorum seeds, and a product derived from the seeds was evaluated. Ethanolic extracts were subjected to bioassays in vitro. The total phenolic content (TPC) was measured by the Folin-Ciocalteu assay, and the seed extract showed a TPC value of 0.58 ± 0.05 GAE/g, while the product showed a TPC of 0.68 ± 0.13 GAE/g (p > 0.05). The total flavonoid content (TFC) was determined by the aluminum chloride colorimetric method, in which the TFC values for the seed extract and the product were 0.12 ± 0.01 QE/g and 0.30 ± 0.06 QE/g, respectively (p < 0.05). The antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, where a maximum radical scavenging value of 70.48% ± 1.86 at 1 mg/mL was found for the seed extract. A similar value was obtained for the product extract; 70.70% ± 0.93 at 1 mg/mL (p > 0.05), while ascorbic acid which was the standard showed 75.55% ± 2.18 (1 mg/mL). The Human Red Blood Cell Membrane Stabilization (HRBC) assay was conducted, and the anti-inflammatory results depicted 78.41% ± 3.13 for the seed extract and 83.16% ± 1.73 for the product extract for 1 mg/mL (p > 0.05). Standard Ibuprofen showed a value of 5-diphenyltetrazolium bromide (MTT) assay against the HepG2 liver cancer cells. The seed extract showed a maximum cancer cell inhibitory value of 37.19% ± 0.80 at 1 mg/mL, and the product showed a value of 40.18% ± 0.90 at 1 mg/mL (p > 0.05). The product generally showed higher bioactivity than the seed extract, although the differences were not always statistically significant. The bioactivity of the derived product is similar to that of the raw seeds, highlighting the potential health benefits of the derived product.
Description
Keywords
Anti-inflammatory , Antioxidant , Cytotoxicity , Strychnos potatorum product , Strychnos potatorum seeds
Citation
Proceedings of the Postgraduate of Institute of Science Research Congress (RESCON) -2024, University of Peradeniya, P 161