Study of callus formation and regeneration of selected rice varieties and transfer of sytheticcry1 Gene rice callus
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Date
2000-12-16
Authors
Wickramaratne, N. A. A. D.
Bandara, J. M. R. S.
Journal Title
Journal ISSN
Volume Title
Publisher
University of Peradeniya
Abstract
Rice (Oryza sativa. L) is one of the most important food crop of the world serving as a major staple food crop for about 3 billion people In developing countries, 35%.55% of the potential yield of rice is lost mainly due to pest and diseases. In South and Southeast Asia, the average yield lost due to insect pest is 18.5%. Mordern methods of important include gene transfer to obtain pest resistant varieties. Tissue culture is a principal tool in gene transformation. In this study callus formation and regeneration of selected rice varieties Bg300, Bg304. Bg380. Bg352, Bg350 & IR8 were examined. Seeds were cultured on R2 medium with two 2. 4-D levels (I mg/l and 2 mg/l). Callus formation percentage was observed after 2 weeks of culturing. calli colour was observed at first subculturing and size was also measured prior to transfer to the regeneration medium Rice calli (Bg352) were co-cultivated with Agerobateriurn.' . bi C\\rBI\ !1()! ith heti I strain C58 carrying recom mant vector p .. . . Wit synt etrc cry gene. Transformation efficiency was checked by the GUS assay. Bg352 was the best in callus formation with favourable colour and favourable size. It is also the quickest to respond bv becoming green when transferred to regeneration medium, , f l 'I'd II' GL'S" T DN'A f 'h C.\.\fB1.\ Fifty three percent 0 t ie co-cu tivate ca I were I positive - 1 part 0 t e p 1.101has integrated successfullv with a genome of Bg352.
Description
Keywords
Agricultural Biology , Rice , Callus formation
Citation
Proceedings and Abstract of the Annual Research Sessions, December 16, 2000, University of Peradeniya, Sri Lanka pp 191-192