Comparative antioxidant activity and quantification of gymnemic acid from Sri Lankan gymnema SP
| dc.contributor.author | Geethika, C. D. H. | |
| dc.date.accessioned | 2024-08-16T07:41:04Z | |
| dc.date.available | 2024-08-16T07:41:04Z | |
| dc.date.issued | 2009 | |
| dc.description.abstract | Gymnema lactiferum (L.) R. Br. ex Schultes in Roemer & Schultes (Sin: Kuringnan; Tam: Kurintai; Eng: Ceylon cow plant) is widely used to treat diabetic patients and other urinary disorders in ayurvedic medicinal formulations in Sri Lanka from ancient times. Also the use of Gymnema sylvestre R. Br. (Sin: Masbedda; Tam: Cherukurinja, Sirukurunkay; Eng: Periploca of the woods) an Indian woody climber has increased recently because of the pharmaceutical potential of its antidiabetic components. Both G. lactiferum (L.) R. Br. ex Schultes in Roemer & Schultes and G. sylvestre R. Br. belong to the Asclepiadaceae family and they show this antidiabetic activity is due to the gymnemic acids present in the leaves. It has also been reported that G. sylvestre contains fairly good antioxidant activities. So far very less information is available on research on G. lactiferum (L.) R. Br. ex Schultes in Roemer & Schultes. Therefore this study focused on the Sri Lankan Gymnema species, G. lactiferum (L.) R. Br. ex Schultes in Roemer & Schultes and G. sylvestre R. Br in comparison to Indian counterpart. In the present study on quantification of gymnemic acid in Gymnema sp. was done by High Performace Liquid Chromatography (HPLC) and Gravimetry. Even though HPLC is an accurate and sophisticated method, gravimetry is a rapid and easy method which can be used to estimate gymnemic acid content in industrial applications. When considering the antioxidant activity of Gymnema sp. DPPH assay was used to evaluate antioxidant potential. This method has been developed to determine the antioxidant activity of different extracts using the stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. It has been observed that Gymnema lactiferum (L.) R. Br. ex Schultes in Roemer & Schultes has a higher gymnemic acid content (94.2%) and a higher antioxidant activity (EC(50): 311.1 ± 29.8), compared to G. sylvestre (Dambulla- EC(50): 478.7 ± 23.8 and(Indian- EC(50) : 463.4 ± 44.0). | |
| dc.identifier.uri | https://ir.lib.pdn.ac.lk/handle/20.500.14444/605 | |
| dc.language.iso | en_US | |
| dc.publisher | University of Peradeniya | |
| dc.subject | Chemical sciences | |
| dc.subject | Analytical chemistry | |
| dc.subject | Gymnemic acid | |
| dc.title | Comparative antioxidant activity and quantification of gymnemic acid from Sri Lankan gymnema SP | |
| dc.type | Thesis |