Bioactive constituents from the Ethyl Acetate extract of the fruits Garcinia cambogia
Loading...
Date
2013-07-04
Authors
De Silva, G. G. E. H.
Kumar, N. S.
Jayasinghe, U. L. B.
Journal Title
Journal ISSN
Volume Title
Publisher
The University of Peradeniya
Abstract
Garcinia cambogia of the family Clusiaceae is a moderate-sized multipurpose tree. It has been used in Sri Lanka from time immemorial for culinary and medicinal purposes. Garciniacambogia is becoming a popular species in the world because of its potential as a dietary supplement for weight loss and appetite control. The objective of the current study was to identify the bioactivity of the ethyl acetate extract and to isolate the bioactive constituents. Fresh fruits of Garcinia cambogia were extracted with hexane, ethyl acetate (EtOAc) and methanol (MeOH). Each extract was subjected to the assay for antioxidant activity against DPPH radicals, phytotoxic activity against Lactuca sativa seed germination, cytotoxic activity against brine shrimp lethality, antifungal activity against Cladosporium cladosporioides (TLC –bio autographic method) and α-amylase enzyme inhibition assay.
Results from the antioxidant activity assay, EtOAc extract showed <formula> at 88 ppm which indicated moderate antioxidant activity when compared to the standards (ascorbic acid <formula> at 6.5 ppm). The EtOAc extract showed 100% root inhibition at 500 ppm and 1000 ppm respectively. EtOAc extract showed <formula> at 200 ppm for brine shrimp lethality assay (artificial sea water was used as the positive control). The α-amylase enzyme inhibition of the EtOAc was <formula> at 1100 ppm (acarbose used as the positive control). EtOAc extract did not show antifungal activity against C. cladosporioides. Chromatographic separation of the ethyl acetate extract furnished six pure compounds. Four of these compounds were identified as garcinol, 7-epi-garcinol, guttiferone I and guttiferone J by comparison of 1H and 13C-NMR spectral data with those reported in the literature. Garcinol, (<formula> -6 ppm, ascorbic acid <formula> 6.5 ppm), 7-epigarcinol (<formula> 4.4 ppm) and guttiferone I (<formula> 25 ppm) showed potent antioxidant activity and only garcinol showed potent brine shrimp lethality (<formula> at 5 ppm). Guttiferone J showed 100% root inhibition of L. sativa at 125ppm
Description
Keywords
Carcinia cambogia , Ethyl acetate , Bioactive constituets
Citation
Peradeniya University Research Sessions PURSE - 2012, Book of Abstracts, University of Peradeniya, Sri Lanka, Vol. 17, July. 4. 2012 pp.172