Chemical Constituents of ๐๐ณ๐ต๐ฐ๐ค๐ข๐ณ๐ฑ๐ถ๐ด ๐๐ญ๐ต๐ช๐ญ๐ช๐ด
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Date
2004-11-10
Authors
Jayasinghe, U. L. B.
Amarasinghe, N. R.
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Publisher
University of Peradeniya Sri Lanka
Abstract
In a continuation of our studies on search for biologically active compounds from Sri Lankan plants the present study was carried out on the fruits of ๐๐ณ๐ต๐ฐ๐ค๐ข๐ณ๐ฑ๐ถ๐ด ๐๐ญ๐ต๐ช๐ญ๐ช๐ด (Park.) Fosb .. ๐. ๐ข๐ญ๐ต๐ช๐ญ๐ช๐ด of the family Moraceae is a tree of moderate size, commonly known as bread fruit growing in tropical countries[The abstract also includes scientific formulas and symbols to represent key concepts and findings].
The dried powdered fruits of ๐๐ณ๐ต๐ฐ๐ค๐ข๐ณ๐ฑ๐ถ๐ด ๐ข๐ญ๐ต๐ช๐ญ๐ช๐ด were defatted with n-hexane and extracted with ethyl acetate and methanol at room temperature. Preliminary investigation indicated the presence of antifungal active compounds against ๐๐ญ๐ข๐ฅ๐ฐ๐ด๐ฑ๐ฐ๐ณ๐ช๐ถ๐ฎ ๐ค๐ญ๐ข๐ฅ๐ฐ๐ด๐ฑ๐ฐ๐ณ๐ช๐ฐ๐ช๐ฅ๐ฆ๐ด in both ethyl acetate and methanol extracts in the same Rf region by TLC bioautography method. In order to isolate antifungal active compounds, both ethyl acetate and methanol extracts were combined and separated by a combination of chromatographies over column of silica gel, Sephadex LH-20, reverse phase silica gel and reverse phase HPLC to give seven compounds. Four of them were identified as (๐)-4-(3-methyl-E-but-1-enyl)-3,5,2', 4'- tetrahydroxystilbene (1), (๐)-3,5,2',4' -tetrahydroxystilbene (2), 2-(2,4-dihydroxyphenyl)- 5,7-dihydroxychroman-4-one (3) and 2-(3,5-dihydroxyphenyl) benzofuran-4, 6-diol (4). Structure elucidation of isolates was based on the detailed analysis of ยนHNMR, ยนยณCNMR, MS and direct comparison of the reported data. All these compounds showed radical scavenging properties towards 2,2' -diphenyl-I-picrylhydrazyl (DPPH) radical by TLC bio-autography method. Structure elucidation of the other three compounds and determination of antioxidant activity (ICโ
โ) and antifungal activity (MIC) of isolates are in progress.
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Citation
Proceedings of the Peradeniya University Research Sessions, 2004, Vol. 9, pp.145