Antigenic analysis of Bovine Sarcocystis SPP in Sri Lanka
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Date
2004
Authors
Hettiarachchi, C.
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Publisher
University of Sri Lanka
Abstract
Sarcocystis is an intracellular parasite, known to cause acute and chronic diseases in a variety of hosts, including cattle. This work was designed to characterize Sarcocystis antigens by molecular weight and to investigate the possibility of cross-reaction with related Apicomplexan T. oxoptasma.gondii. Sarcocystis antigen was prepared by chemical lysis (10% Triton) of bradyzoites contained in tissue cysts. Healthy rabbits were inoculated with Sarcocystis (antigen and whole parasite) and Toxoplasma and antibodies harvested after about a month’s lapse. A higher antibody titer was elicited by the inoculation of Sarcocystis cystozoites as opposed to the antigen. The latex agglutination test confirmed presence of anti Toxoplasma antibodies in test serum and negated cross-reactivity between Sarcocystisand Toxoplasma at 1:100 serum dilution. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and subsequent immunoblot analysis at 1:100 serum dilution revealed twelve antigenic bands, three of which were prominent. Out of these three, two were complex bands of ~ 66 kMW and above. The other was a single band of ~ 45 kMW. Monoclonal antibodies can be developed against these potent antigens for diagnostic purposes in the future. Immunoblot results also dismissed cross- reactivity. A highly sensitive assay should be developed to avoid ambiguity in routine diagnostic tests.
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Biochemistry & Molecular Biology , Sri Lanka