Absence of dengue virus NS 1 Antigen in Aedes aegypti and Aedes albopictus mosquitoes collected from urban areas of Peradeniya and Kegalle
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Date
2013-07-04
Authors
Ekiriyagala, W. R. S. K.
Noordeen, F.
Abeykoon, A. M. S. B.
Ariyaratne, C. S.
Journal Title
Journal ISSN
Volume Title
Publisher
The University of Peradeniya
Abstract
Dengue fever (DF) is an important mosquito-borne disease that affects humans causing morbidity and mortality. With neither vaccines nor treatment available, prevention of the disease relies heavily on surveillance and control of mosquito vectors. Aedes aegypti is considered to be the major vector for dengue virus (DENV) transmission, whereas Aedes albopictus is considered to be the secondary vector. Both Aedes spp. are responsible for carrying any of the four serotypes of DENVs (DENV1, DENV2, DENV3, and DENV4).
Firstly, we examined the Aedes mosquitoes collected outdoors to identify which of the Aedes species was abundant in the urban areas of Kegalle and Peradeniya. Secondly, we evaluated NS1 antigen detection (SD BIOLINE Dengue NS1 test) in field caught Aedes aegypti and Aedes albopictus mosquitoes (n=165) to gather evidence for DENV carriage.
Aedes albopictus was more abundant (115/165; 70%) than Aedes aegypti (50/165;30%) in the urban areas of Kegalle and Peradeniya. However, we were unable to detect Dengue NS1 antigen in the field caught Aedes aegypti and Aedes albopictus mosquitoes.
The reason for not detecting the Dengue NS1 Ag in the field caught Aedes spp. May be twofold. First, these field caught mosquitoes may not be carrying the DENV or our test was unable to detect the low level of DENV found in the field- caught mosquito pools. However, supernatants obtained from the same mosquito pools that were tested for Dengue NS1 antigen are stored at -80oC for DENV RNA extraction and RT-PCR to confirm the validity of Dengue NS1 antigen detection test in the field caught mosquitoes.
Description
Keywords
Health and hygiene , Dengue fever , Aedes aegypti , Aedes albopictus
Citation
Peradeniya University Research Sessions PURSE - 2012, Book of Abstracts, University of Peradeniya, Sri Lanka, Vol. 17, July. 4. 2012 pp. 134