iPURSE 2019

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https://ir.lib.pdn.ac.lk/handle/20.500.14444/37

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Browsing iPURSE 2019 by Author "Amarasinghe, A. A. P. B. N."

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    Candida species distribution, genotyping and enzyme production of C. albicans isolated from denture stomatitis patients and healthy individuals
    (University of Peradeniya, 2019-09-12) Jayatilake, J. A. M. S.; Amarasinghe, A. A. P. B. N.; Muhandiram, M. R. S.; Kodithuwakku, S. P.
    Denture stomatitis (DS) associated with Candida is common among removable denture wearers. This study investigated species distribution of oral Candida isolated from DS patients and healthy individuals. Furthermore, phospholipase, esterase and hemolysin production by oral C. albicans from DS and healthy individuals were evaluated. Candida isolates from DS patients (n=38) and a comparable healthy group (n=23) were speciated using chrome agar, ABC genotyping and PCR-RFLP. Enzyme production by C. albicans was evaluated using culture plate assays. Chrome agar test revealed 18 C. albicans and 05 non C. albicans isolates in healthy group. In DS group, there were 29 C. albicans and 09 non C. albicans isolates. According to ABC genotyping, C. albicans genotype A was the commonest genotype (66.8%) followed by genotypes C (21.1%) and B (13.1%) among DS patients. The 23 Candida isolates from healthy individuals consisted of genotype A (78.3%) followed by genotypes C (17.4%) and B (4.3%). PCR-RFLP showed that C. albicans (76.3%) was the commonest species followed by C. glabrata (13.2%), C. parapsilosis (5.2%) and C. tropicalis (5.2%) among DS patients. In healthy group, the commonest species was C. albicans (78.3%) followed by C. parapsilosis (8.7%), C. glabrata (4.3%), C. tropicalis (4.3%) and C. guilliermondii (4.3%). Phospholipase, esterase and hemolysin production by C. albicans from DS patients was significantly higher (P<0.05) than C. albicans from healthy individuals. Foregoing data confirms that C. albicans is the leading Candida spp. isolated from DS patients and healthy individuals. Non C. albicans Candida are isolated from both groups occasionally. Considering the current sample, C. albicans genotype A remains the predominant genotype followed by genotypes C and B in both DS patients and healthy individuals. C. albicans from DS patients have significantly higher activities of phospholipase, esterase and haemolysin compared to commensal C. albicans indicating their contribution in pathogenesis of DS.