Bioactive extracts from fruits of Manilkara zapota
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Date
2013-07-04
Authors
Ganegoda, W. M. A. S.
Padmathilake, K. G. E.
Kumar, N. S.
Jayasinghe, U. L. B.
Journal Title
Journal ISSN
Volume Title
Publisher
The University of Peradeniya
Abstract
Manilkarazapota (local name - Sapodilla), belonging to the family Sapotaceae, is a tropical fruit native to Yucataan and nearby parts of southern Mexico and Central America. M. zapota is a yellow brown colour fruit which is ellipsoidal in shape having a soft pulp and containing about 3-5 seeds. The fruit was found to contain high amounts of carbohydrates, saponins and antioxidant polyphenolic compounds such as tannins (proanthocyanidins) which has extreme astringent properties. Earlier research studies have revealed that fruits and leaves of M. zapota show antifungal effects and antibacterial activity and also that the fruit has a strong nitric oxide scavenging activity and inhibitory effects against tumour cell proliferation. Few studies have been reported regarding the bioactivity of fruits of M.zapota, owing to the fact that Sapodilla is a rare fruit tree.
Ripe fruits of M. zapota were collected from Kandy district, Central Province of Sri Lanka in April 2012. The edible parts of the fruits (400 g) were cut in to small pieces and sequentially extracted at room temperature with Hexane, EtOAc and MeOH (30 min x 3) using a sonicator. The extracts were filtered and the filtrates were evaporated under reduced pressure (rotary evaporator) to obtain the Hexane extract (1.1 g), EtOAc extract (3.4 g) and MeOH extract (38.0 g).
All the crude extracts obtained were subjected to bioassays for antioxidant free radical scavenging activity against DPPH (2,2’-diphenyl-1-picrylhydrazyl) radical with spectrophotometric method; brine shrimp lethality test against Artemiasalina using micro-well bioassay and α-amylase inhibition activity using a spectrophotometric method. BHA was used as the standard for the antioxidant assay and Acarbose was used as the standard for the α- amylase inhibition bioassay.
None of the extracts of M. zapota showed significant toxicity to brine shrimps. The MeOH extract of M. zapota showed an IC50 value of 2375 ppm for the α-amylase inhibition activity. Both EtOAc extract and MeOH extract were antioxidant active showing <formula> values of 1500 ppm and 775 ppm respectively.
Description
Keywords
Biological activity , Manilkara zapota , Sapodilla , Antioxidant polyphenolic compounds
Citation
Peradeniya University Research Sessions PURSE - 2012, Book of Abstracts, University of Peradeniya, Sri Lanka, Vol. 17, July. 4. 2013 pp.289