Low cost micropropagatoin for Its domestication
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Date
1998-11-07
Authors
Peiris, S. E.
Peiris, B. C. N.
Journal Title
Journal ISSN
Volume Title
Publisher
University of Peradeniya
Abstract
Micropropagation in vitro requires aseptic environment and sterilized cultures.
Equipment such as autoclaves and laminar airflow cabinets are used for this purpose.
These two pieces of equipment alone cost Rs. 300,000-500,000 plus their running cost.
This unbearable cost hinders the use of micropropagation for planting material
production.
A method of micropropagation was developed in the tissue culture laboratory of
the Crop Science Department, Faculty of Agriculture to overcome the constraints of
using these expensive pieces of equipment. In this method two chemical solutions,
CSUPW and CSUPM, were used for sterilization of glassware and culture media,
respectively. These solutions were tested on orchid seed germination, orchid seedling
growth and anthurium multiplication under in vitro condition.
The jam jars used for orchid seed germination cultures were sterilized with 10%
CSUPW solution and the medium with the CSUPM solution in concentrations of 0, 1,
2.5, 5 and 10%. Non sterilized, as well as autoclaved culture media were used as
controls. Orchid (Dendrobium spp) seeds were established, in the media sterilized in
the above manner, on a laboratory bench. The laminar flow environment was used for
autoclaved culture media. Orchid seedlings in 1 and 2 em sizes were cultured, in test
tubes sterilized with CSUPW solution in 5,10,15 and 20% concentrations, and in the
autoclaved medium as a control. Anthurium plants in 1, 2 and 3 ern sizes were cultured
on an anthurium multiplication medium, which contained 1% CSUPM solution. The
jam jars used for this were sterilized using 5,10, 15 and 20% CSUPW solutions. An
autoclaved medium was used as a control. Storability of the both solutions were also
examined.
All jars which sterilized with 10% CSUPW gave 100% contamination free
cultures. The jars which contained 1% CSUPM solution and the autoclaved cultures
gave 100% orchid seed germination and cultures with 2.5, 5 and 10% CSUPM
solutions had 70, 40 and 30% germination, respectively. Orchid seedling media
sterilized with 5% CSUPW gave 30% contaminated cultures. All other orchid seedling
media were 100% contamination free. Anthurium cultures, when sterilized with 5%
CSUPW solution, had 40% contaminated cultures. All other anthurium cultures were
contamination free. However, lcm anthurium plants in the jars sterilized with 20%
CSUPW had toxic appearance. The solutions gave the same results after storing 3
months.
The solutions, 10-15% CSUPW and 1% CSUPM, developed in this study can
be used to replace high cost equipment such as autoclaves and laminar air flow cabinets
for micropropagation to obtain contamination free cultures.
Description
Keywords
Micropropagation , Domestication , Sterlilized Cultures , Agriculture and animal sciences
Citation
Proceedings & Abstracts of the Annual Research Sessions November 07, 1998,University of Peradeniya,Peradeniya, Sri Lanka,pp.04