Antibacterial activity of selected seaweed extracts against some selected infectious pathogens
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Date
2019-09-12
Authors
Maria Nimalini, M.
Vasantharuba, S.
Kapilan, R.
Journal Title
Journal ISSN
Volume Title
Publisher
University of Peradeniya
Abstract
The antibacterial efficacy of methanolic extracts of marine algae, Caulerpa racemosa (Chlorophyta), Sargassum polycystum and Turbinaria ornata (Phaeophyta) against some selected gram-positive and gram-negative human pathogenic bacteria was studied. Dried and powdered sample (3g) of the seaweeds collected at the Nachchikuda and Keerimalai coastal area in the Northern region of Sri Lanka was diluted with 60 ml 70% methanol. This mixture was homogenized at different duration and then filtered by using whatsman No 1 filter paper to obtain clear extract. This extract was used to test the antibacterial activity against appropriately diluted Gram-positive bacterial species Enterococcus faecalis (ATCC 29212) & Staphylococcus aureus (ATCC 29213) and Gram-negative bacterial species Escherichia coli (ATCC 25922) & Pseudomonas aeroginosa (ATCC 27853) using agar well diffusion method in triplicates. Results showed that significant amount of inhibition zone was obtained against all the tested bacterial species which was comparable with positive control streptomycin and negative control 70% methanol. Turbinaria ornata have significantly highest antibacterial activity against all test organisms followed by Caulerpa racemosa and Sargassum polycystum. The selected seaweeds showed their highest and lowest inhibition zone against Staphylococcus aureus (19.06±1.15mm) and Enterococcus faecalis (12.32±0.76mm).
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Keywords
Antibacterial activity , Methanolic extracts , Pathogens , Seaweeds