Current status of insecticide resistance in mosquito vectors of dangerous human diseases in Sri Lanka
| dc.contributor.author | Karunaratne, S. H. P. P. | |
| dc.date.accessioned | 2024-03-05T05:05:24Z | |
| dc.date.available | 2024-03-05T05:05:24Z | |
| dc.date.issued | 1998-11-07 | |
| dc.description.abstract | Resistance levels to synthetic insecticides and underlying resistance mechanisms were investigated in six species of mosquito vectors. Anophelus cu/icifacies and An. subpictus (vectors of malaria) were collected from Galewela, Matale using bovine baited trap huts. Culex tritaeniarhynchus and ex ge/idus (vectors of Japanese encephalitis) were from Anuradhapura using CDC light traps hung in piggeries. Aedes aegypli and Ae. albopictus (vectors of dengue) were from Kandy by human baited catches. Adult mosquitoes were exposed to insecticide impregnated papers using World Health Organisation bioassay test kits and log-probit mortality curves were established. Activity levels of carboxylesterases, glutathione-Sstransferases and oxidases, which metabolize insecticides before they reach the insect target sites, were investigated using biochemical assays. Quantitative changes of carboxylesterases were detected by native polyacrylamide gel electrophoresis. Qualitative changes were studied by detecting the rates of malathion metabolism. Resistance of the insect target site, acetylcholinesterase, to insecticide inhibition was tested biochemically. Very high resistance to the organophosphate malathion was shown by An. cuiicifacies (70% of the population) and ex tritaeniorhynchus (65%). High resistance to the carbamate propoxur was shown by ex. tritaeniorhynchus (53%) and Ae. aegypti (44.5%). Anopheline and Aedes populations showed a low resistance to the pyrethroid pennethrin (-25%). All the species, except C. ge/idus were highly resistant to DDT (62.4% - 100%). Resistance of all six populations to newly introduced pyrethroids ie. deltamethrin, cypermethrin and lambda cyhalothrin was negligible (0% - 18%). Acetylcholinesterase, the target site of organophosphates and carbamates, is altered in a high proportion of all mosquito populations. Glutathione-Svtransferases and oxidases significantly contribute to the resistance shown by anopheline vectors. Elevation (qualitative changes) of carboxylesterases was not detected only in An. subpictus and C. gelidus. Presence of qualitatively different carboxylesterases, which metabolize insecticides at a faster rate, was found only in anopheline populations . | |
| dc.identifier.citation | Proceedings & Abstracts of the Annual Research Sessions,1998,University of Peradeniya, peradeniya, Sri Lanka, pp 23 | |
| dc.identifier.issn | 1391-4111 | |
| dc.identifier.uri | https://ir.lib.pdn.ac.lk/handle/20.500.14444/164 | |
| dc.language.iso | en_US | |
| dc.publisher | University of Peradeniya | |
| dc.subject | Biological sciences | |
| dc.subject | Insecticide resistance | |
| dc.subject | Mosquito vectors | |
| dc.subject | Human Dieseases | |
| dc.title | Current status of insecticide resistance in mosquito vectors of dangerous human diseases in Sri Lanka | |
| dc.type | Article |