Antimicrobial Activity of Some Sri Lankan Rubiaceae and Meliaceae
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Date
2001-11-16
Authors
Jayasinghe, U. L. B.
Jayasooriya, C. P.
Bandara, B. M. R.
Ekanayake, S. P.
Merlini, L.
Assante, G
Journal Title
Journal ISSN
Volume Title
Publisher
University of Peradeniya, Peradeniya, Sri Lanka
Abstract
Continuing our work on biologically active compounds from Sri Lankan plants we have
now screened 94 solvent extracts (n-hexane, dichloromethane and methanol) obtained from
leaves, bark and stem of thirteen Rubiaceae species (Benkara malabarica, Canthium
coromandelicum, Canthium dicoccum, Haldina cordifolia, Ixora calycina, Morinda tinctoria,
Mussaendafrondosa, Psychotria gardneri, Psychotria nigra, Psycho tria stenophylla, Saprosma
foetens, Tarenna asiatica and Wendlandia bicuspidatay and two Meliaceae species (Aglia
congylos and Munronia pumilia) for antibacterial activity against Escherichia coli (Ec),
Micrococcus luteus (Ml), Bacillus subtilis (Bs) and Bacillus cereus (Bc) and antifungal activity
against Saccharomyces cerevisiae (Se), Ustilago maydis (Um) and Aspergillus niger (An).
Screening for antimicrobial activity was carried out using the disc diffusion method. The media
used for culturing bacteria, fungi and yeast like fungi were nutrient agar, Czapek agar and
glucose yeast agar, respectively.
The plants M.tinctoria, M.frondosa, P. gardneri and P. stenophylla showed
antimicrobial activity against at least five of the seven microbes examined: M. tinetoria (leaves)
- hexane extract against Ec, Bs, Be and An, and dichloromethane extract against Sc, Ec and Be;
M tinetoria(bark) - dichloromethane extract against Se, Ec, Bs and An, and methanol extract
against MI and Be; M frondosa (leaves) - dichloromethane extract against Sc, Ec, Bs and Be,
and methanol extract against Bs; M frondosa (bark) - hexane extract against Sc, Um, Ml and
Be, dichloromethane extract against Se, MI and Be, and methanol extract against Ec and Bs; P.
gardneri (leaves) - dichloromethane extract against An,. and methanol extract against Sc, £C, Ml
and Be; P. gardneri (bark) - hexane extract against An, dichloromethane extract against Se and
Bs, and methanol extract against Ec, MI, Bs and Be; P. stenophylla (leaves) - dichloromethane
extract against Ec; P. stenophylla (bark) - dichloromethane extract against Um, £C, MI, Bs, Be
and An and methanol extract against MI. The following extracts were found to be inactive: A.
congylos (leaves - methanol), B. malabarica (leaves - hexane), C. coromandelicum (leaves -
hexane), H. eordifolia (leaves -hexane, bark - hexane and methanol), I. calycina (bark -
hexane), M tinetoria (leaves-methanol, bark - hexane), M. frondosa (leaves - hexane), M
puma/ia (leaves - hexane, dichloromethane and methanol), P. gardneri (leaves - hexane), P.
nigra (leaves - hexane and dichloromethane), P. stenophylla (leaves - hexane and methanol,
bark - hexane), S. foetens (bark - hexane), T asiatica (bark - hexane), W bicuspidata (leaves -
methanol, bark - methanol).
Description
Keywords
Antimicrobial , Science , Activity , Rubiaceae , Meliaceae
Citation
Proceedings & abstracts of the Annual Research Sessions 2001,University of Peradeniya, Peradeniya, Sri Lanka,pp.110